Food (nutritional composition) testing

This standard specifies the determination method for niacin and nicotinamide in food. The first method of this standard is the microbiological method, which is applicable to the determination of the total amount of niacin and niacinamide in various foods, including fortified foods based on natural foods; The second method is high-performance liquid chromatography, which is suitable for the determination of niacin and niacinamide in fortified foods. The first microbiological method 2 principle is that niacin (niacinamide) is an essential nutrient for the growth of Lactobacillus plantarum (ATCC 8014). Under certain control conditions, using the specificity of Lactobacillus plantarum for niacin and niacinamide, the content of niacin and niacinamide is determined by the optical density formed by the growth of samples containing niacin and niacinamide

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