Quality inspection

National Food Safety Standard for the Determination of Fatty Acids in Food 1 Scope This standard specifies the method for determining the content of fatty acids in food. This standard is applicable to the determination of total fat, saturated fat (acid), and unsaturated fat (acid) in food. The hydrolysis extraction method in this standard is applicable to the determination of fatty acid content in food; The ester exchange method is applicable to the determination of fatty acid content in oil samples with a free fatty acid content not exceeding 2%; The acetyl chloride methanol method is suitable for the determination of fatty acid content in milk powder and anhydrous cream samples with a water content of less than 5%. The first method, Internal Standard Method 2 Principle 2.1 Hydrolysis Extraction Method: After adding an internal standard, the sample is subjected to hydrolysis ether solution to extract the fat, which is then saponified and methylated under alkaline conditions to generate fatty acid methyl ester. The content of fatty acid methyl ester is quantitatively determined by capillary column gas chromatography and internal standard method. Calculate the total fat, saturated fat (acid), monounsaturated fat (acid), and polyunsaturated fat (acid) content based on the methyl ester content and conversion coefficient of various fatty acids. Animal and vegetable oil samples do not undergo fat extraction, but are directly saponified and fatty acid methylated after adding internal standards. 2.2 Ester exchange method (applicable to oils with a free fatty acid content of no more than 2%): Dissolve the oil in isooctane, add an internal standard, and then add potassium hydroxide methanol solution for ester exchange methylation. After the reaction is complete, neutralize the remaining potassium hydroxide with sodium bisulfate to avoid methyl ester saponification. Unless otherwise specified, all reagents and materials used in this method are analytical pure, and the water is the first grade water specified in GB/T 6682

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