Lactose detection

This standard specifies the determination method of lactose and fructose in infant and young child food and dairy products. This standard is applicable to the determination of lactose and sucrose in infant food and dairy products. 2 Normative References The documents referenced in this standard are essential for the application of this standard. For dated references, only the version with the date indicated applies to this standard. For undated references, the latest version (including all amendments) applies to this standard. The first method is the principle of high-performance liquid chromatography. After extraction, lactose and sucrose in the sample are separated using a high-performance liquid chromatography column and detected using a differential refractive detector or evaporative light scattering detector. The external standard method is used for quantification. Unless otherwise specified, all reagents and materials used in this method are analytical pure, and the water is Grade 1 water as specified in GB/T 6682. 4.1 Acetonitrile. 4.2 Acetonitrile: Chromatically pure. 4.3 Standard solution 4.3.1 Lactose standard stock solution (20 mg/mL): Weigh 2 g of lactose standard sample (accurate to 0.1 mg) that has been dried in a 94 ℃ soil 2 ℃ oven for 2 hours, dissolve it in water, and dilute with water to a 100 mL volumetric flask. Place in a 4 ℃ refrigerator. 4.3.2 Lactose standard working solution: Take 0 mL, 1 mL, 2 mL, 3 mL, 4 mL, and 5 mL of lactose standard stock solution (4.3.1) into a 10 mL volumetric flask, and bring to volume with ethylene wax (4.1) to the mark. Prepare a series of lactose standard working solutions with concentrations of 0 mg/mL, 2 mg/mL, 4 mg/mL, 6 mg/mL, 8 mg/mL, and 10 mg/mL. 4.3.3 Sucrose standard solution (10 mg/mL): Weigh 1gK (accurate to 0.1 mg) of sucrose standard sample dried in a 105 ℃ soil 2 ℃ oven for 2 hours, dissolve it in water, and dilute with water to a 100mL volumetric flask. Place in a 4 ℃ refrigerator. 4.3.4: Sucrose standard working solution: Take 0 mL, 1 mL, 2 mL, 3 mL, 4 mL, and 5 mL of sucrose standard solution (4.3.3) into a 10 mL volumetric flask, and bring to volume with acetonitrile (4.1) to the mark. Prepare sucrose standard series working solutions with concentrations of 0 mg/mL, 1 mg/mL, 2 mg/mL, 3 mg/mL, 4 mg/mL, and 5 mg/mL

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