Spore powder detection

The methods of spore powder testing involve multiple aspects to ensure its quality, safety, and active ingredient content. The following are some common spore powder testing methods:

1. Appearance testing
Evaluate the appearance characteristics of spore powder such as color, smell, texture, etc. through visual inspection and olfaction. High-quality spore powder usually has a uniform color, no obvious impurities, a natural woody aroma, and a fine texture.

2. Active ingredient testing
Polysaccharide content testing:
Polysaccharides are important active ingredients in spore powder, which have immune enhancement and antioxidant effects.
The determination of polysaccharide content is usually carried out by the phenol-sulfuric acid method or high-performance liquid chromatography (HPLC).
Triterpenoid content testing:
Triterpenoids are also key active ingredients in spore powder, with anti-inflammatory, anti-tumor, and liver protection effects.
The determination of its content is usually carried out by HPLC or gas chromatography (GC).
Protein content testing:
Protein is an important nutrient in spore powder and has multiple biological activities.
The determination of protein content can be carried out by methods such as Kjeldahl nitrogen determination and ultraviolet spectrophotometry.
Wall breaking rate detection:
The wall-breaking rate is an important indicator to measure the quality of spore powder because the wall-breaking treatment can improve the bioavailability of the effective ingredients in the spore powder.
The wall-breaking rate can be detected by microscopic observation, electron microscopic analysis, or enzymatic hydrolysis.
3. Impurity and purity detection
Ash content detection:
Ash content reflects the inorganic content and impurity content in the spore powder.
The ash content is usually determined by the high-temperature ashing method.
Moisture content detection:
The moisture content is an important indicator to measure the dryness of spore powder, which affects the shelf life and stability of active ingredients.
The moisture content can be determined by the drying method, infrared drying method, or Karl Fischer method.
4. Microbial detection
Microbial detection mainly evaluates the content of microorganisms such as bacteria, molds, yeasts, etc. in spore powder to ensure its food safety. Common microbial detection methods include the plate counting method, polymerase chain reaction (PCR) method, etc.

5. Heavy metal and pesticide residue detection
Heavy metal content detection:
Heavy metal content is an important indicator to measure the safety of spore powder.
The determination of heavy metal content can be carried out by atomic absorption spectroscopy, inductively coupled plasma mass spectrometry (ICP-MS), and other methods.
Pesticide residue detection:
Pesticide residue detection is a key step to ensure the safety of spore powder, especially when pesticides may be used during the planting process.
Pesticide residue detection can be carried out by gas chromatography, liquid chromatography gas chromatography-mass spectrometry (GC-MS), and other methods.
VI. Other tests
Mycotoxin detection:
Mycotoxins are toxic metabolites produced by certain fungi, which directly affect the edible safety of spore powder.
Mycotoxin detection can be carried out by high-performance liquid chromatography, liquid chromatography-mass spectrometry (HPLC-MS), and other methods.
Environmental pollutant detection:
Mainly detect whether spore powder contains environmental pollutants such as polycyclic aromatic hydrocarbons and dioxins.
These pollutants can be detected by HPLC, GC-MS, and other methods.
Radioactive material detection:
Ensure that spore powder is not contaminated by radioactivity, especially spore powder planted in certain highly polluted areas or special environments.
Radioactive material detection can be carried out by gamma spectrometer, liquid scintillation counting, and other methods.
Precautions
Before testing, ensure that the testing equipment and samples are fully prepared and meet relevant standards and requirements.
During the testing process, the operating procedures and safety specifications should be strictly followed to prevent contamination and cross-contamination.
For different types of spore powder, appropriate testing methods and steps should be selected according to their characteristics and requirements.
In summary, the spore powder testing method involves multiple aspects and links, and it is necessary to comprehensively use a variety of testing technologies and means to ensure its quality, safety, and active ingredient content.