Pig blood test

The method of pig blood testing is mainly determined by the purpose of the test and the items to be tested. The following are some common methods for pig blood testing:

1. Serum separation and testing
Sample collection:
Collection site: Usually the anterior vena cava or ear vein is selected for blood collection.
Collection method: After the pig is restrained with a restraining rope, blood is collected with a disposable blood collector or syringe. After blood collection, press with a cotton ball to stop bleeding and disinfect.
Number of samples: Determine the number of samples per group according to the size of the pig farm, for example, 5 samples per group for a farm with less than 300 sows, 7 samples per group for a farm with 300 to 700 sows, and so on.
Serum separation:
Put the collected blood sample in a sterile test tube and let it stand or centrifuge to separate the serum.
The serum should be divided into two equal parts, one as a test sample and the other as a backup frozen storage.
Detection method:
According to the specific test items, such as antigens, antibodies, pathogens, etc., select appropriate detection methods, such as enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR), etc.
Follow the kit instructions or laboratory standard operating procedures for testing.
2. Whole blood test
For some test items, such as red blood cell morphology, white blood cell count, etc., whole blood can be used directly for testing. Testing methods include microscopic observation, blood cell analyzer, etc.

3. Other testing methods
Pathogen detection:
For pigs suspected of being infected with specific pathogens, blood samples can be collected for pathogen detection, such as bacterial culture, virus isolation, etc.
Drug residue detection:
For pigs that have used drugs, blood samples can be collected for drug residue detection to ensure the safety of pork.
Serological testing:
By detecting the antibody level in the serum, the immune status or infection history of the pig can be evaluated.
4. Precautions
Sample collection and processing:
When collecting samples, the sterile operation should be ensured to avoid cross-contamination.
The collected samples should be processed in time to avoid long-term storage leading to deterioration or contamination.
Testing reagents and equipment:
Use high-quality testing reagents and equipment to ensure the accuracy of the test results.
Follow the kit instructions or laboratory standard operating procedures for testing to avoid false positive or false negative results caused by operating errors.
Interpretation and reporting of results:
Based on the test results, make a comprehensive judgment in combination with clinical symptoms and epidemiological data.
Report the test results in a timely and accurate manner to provide scientific basis for veterinarians or relevant decision makers.
In summary, there are many methods for pig blood testing, and the appropriate test method should be selected according to the specific test items and needs. At the same time, the principle of aseptic operation should be strictly followed during the test process to ensure the accuracy and reliability of the test results.