Food (sorbic acid, benzoic acid) testing

Sodium benzoate, potassium sorbate, and saccharin sodium are widely used as food additives in food production and processing. This type of indicator is often involved in daily sampling and monitoring, covering a wide range of varieties, and there are often unqualified information released. The national food safety standard for detecting benzoic acid, sorbic acid, and saccharin sodium is GB 5009.28-2016. The standard was officially implemented on June 23, 2017, and the methods include liquid chromatography and gas chromatography. However, the methods involve multiple matrices and there is no purification step in sample pretreatment. If there is insufficient experience or familiarity with various operating points during the inspection, there may be a risk of misjudgment or omission. Therefore, this article mainly interprets the scope of application, basic principles, operational considerations, and results judgment considerations of this method. The scope of application of the standard specifies the method for determining benzoic acid, sorbic acid, and saccharin sodium in food. The first method (liquid chromatography) is applicable to the determination of benzoic acid, sorbic acid, and saccharin sodium in food; The second method (gas chromatography) is applicable to the determination of benzoic acid and sorbic acid in soy sauce, fruit juice, and jam. There are differences in the scope of application between the first and second laws. Basic Principle First Method (Liquid Chromatography): Samples are extracted with water, high fat samples are defatted with n-hexane, high protein samples are precipitated with protein precipitant, separated by liquid chromatography, detected by UV detector, and quantified by external standard method. The second method (gas chromatography): After the sample is acidified with hydrochloric acid, benzoic acid and sorbic acid are extracted with ether, separated and determined using gas chromatography hydrogen flame ionization detector, and quantified using external standard method

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