Veterinary (Animal Quarantine) Testing

This standard specifies the technical requirements for virus isolation, identification, and disease diagnosis methods for porcine circovirus disease. This standard is applicable to the monitoring, quarantine, diagnosis, and epidemiological investigation of porcine circovirus disease. The following normative references are essential for the application of this document. For dated references, only the dated version applies to this document. For undated references, the latest version (including all modification orders) applies to this document. GB/T 6682 Analytical Laboratory Water Specifications and Test Methods 3 Abbreviations The following abbreviations apply to this document. Ct value: number of cycles experienced when the fluorescence signal in each reaction tube reaches the set threshold CPE: cytopathic effect DNA: deoxyribonucleic acid FITC: isothiocyanate fluorescent yellow IFA: indirect immunofluorescence test MEM: minimum requirement culture medium Marker: molecular weight marker nPCR: nested polymerase chain reaction PCV1: porcine circovirus type 1 PCV2: porcine circovirus type 2 PK-15: porcine kidney passage cell line PBS: phosphate buffer Real time PCR: fluorescent polymerase chain reaction Reaction SDS: Sodium dodecyl sulfonate Taq enzyme: Taq DNA polymerase

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