Health food parameter 450671 detection

This standard specifies aflatoxin B, aflatoxin B, and aflatoxin G in food;, Method for the determination of aflatoxin G (hereinafter referred to as AFT B, AFT B, AFT G, and AFT G). The first method of this standard is isotope dilution liquid chromatography tandem mass spectrometry, which is applicable to the determination of AFT B, AFT B, AFTG, and AFT G in grains and their products, beans and their products, nuts and seeds, oils and their products, seasonings, infant formula foods, and infant auxiliary foods. The second method of this standard is the high-performance liquid chromatography pre column derivatization method, which is applicable to the determination of AFT B, AFT B, AFT G, and AFT G in grains and their products, beans and their products, nuts and seeds, oils and their products, seasonings, infant formula foods, and infant auxiliary foods. The third method of this standard is the high-performance liquid chromatography post column derivatization method, which is applicable to the determination of AFT B, AFT B, AFTG, and AFT G in grains and their products, beans and their products, nuts and seeds, oils and their products, seasonings, infant formula foods, and infant auxiliary foods. The fourth method of this standard is the enzyme-linked immunosorbent assay (ELISA) screening method, which is applicable to the determination of AFTB in grains and their products, legumes and their products, nuts and seeds, oils and their products, seasonings, Yan Preschool formula foods, and supplementary foods for infants and young children. The fifth method of this standard is thin layer chromatography, which is applicable to the determination of AFTB in grains and their products, beans and their products, nuts and seeds, oils and their products, and seasonings. The aflatoxin B, aflatoxin B, aflatoxin G, and aflatoxin G in the 2-principle sample were extracted with acetonitrile aqueous solution or methanol aqueous solution. The extracted solution was diluted with a phosphate buffer solution containing 1% Triton X-100 (or Tween-20) (initially purified by a aflatoxin solid-phase purification column if necessary), purified and enriched by an immunoaffinity column. The purified solution was concentrated, volumetric, and filtered before being separated by liquid chromatography and detected by tandem mass spectrometry, Quantitative analysis using isotope internal standard method. Unless otherwise specified, all reagents and materials used in this method are analytical pure, and the water is the first grade water specified in GB/T 6682. 3.1 Reagent 3.1.1 Acetonitrile (CH, CN): Chromatically pure. 3.1.2 Methanol (CH, OH): Chromatically pure. 3.1.3 Ammonium acetate (CH, cOONH): color harmonic purity. 3.1.4 Sodium chloride (NaCl)

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