Amino acid testing

The Research Institute of BAIJIAN focuses on the field of amino acid analysis and has a high-speed amino acid analyzer that can perform methodological validation analysis of amino acids in pharmaceutical related products. Suitable for food, feed, fertilizer and other fields, welcome to submit for inspection and testing. Analyze and test 17 types of amino acids, which can be tested for hydrolysis or free state. Please consult online engineers for details

The Baijianwei Research Institute referred to the national standard GB/T 5009.124-2003 for the determination of amino acids in food. The sample protein was hydrolyzed by hydrochloric acid to form free amino acids, which were separated by the ion exchange column of the amino acid automatic analyzer. After color reaction with the ninhydrin solution, the amino acid content was determined by spectrophotometer colorimetry. Development and validation of analytical methods for 16 amino acids including aspartic acid, threonine, serine, etc

Amino acid detection range:

Serial number

Amino acid name

Serial number

1

Aspartate ASP10

10

Isoleucine ILE

2

Threonine THR11

Leucine LEU

3

Serine SER12

12

Tyrosine TYR

4

Glutamic acid GLU13

13

Phenylalanine PHE

5

Glycine GLY14

Lysine LYS

6

Alanine ALA15

15

Histidine HIS

7

Cystine CYS 16

16

Arginine ARG

8

Valine VAL17

17

Proline PRO

9

Methionine MET

1. Weigh the sample

After crushing, accurately weigh the sample with an electronic balance to the nearest 0.0001g (within the protein content range of 10mg~20mg), and place the weighed sample in a hydrolysis tube

2. Hydrolysis

Add 15mL of 6mol/L hydrochloric acid to the hydrolysis tube, 3-4 drops of newly distilled phenol, and then place the hydrolysis tube in ice water, freeze for 3-5 minutes, and connect it to the vacuum pump's suction pipe. After vacuuming, flush in high-purity nitrogen gas; Vacuum and fill with nitrogen again, repeat three times, and seal in a nitrogen filled state. Place the sealed hydrolysis tube in a constant temperature drying oven at 110 ℃ and 1 ℃. After hydrolysis for 22 hours, remove and cool it

Open the hydrolysis tube, filter the hydrolysate, rinse the tube multiple times with deionized water, transfer all the hydrolysate to a 50mL volumetric flask, dilute with deionized water, draw 1mL of the filtrate into a 5mL volumetric flask, dry with a vacuum dryer at 40 ℃~50 ℃, dissolve the residue in 1-2 mL of water, and then dry again. Repeat twice before evaporating to dryness. Dissolve in 1mL of pH2.2 buffer solution for instrument measurement

Case study

Instrument: Hitachi L-8900 high-speed amino acid analyzer

Post column derivatization, derivative reaction temperature 135 ° C

Atlas case study, 17 amino acids detailed in the atlas

Number

Standard

Name

1

LC-MS laboratory internal method

Laboratory internal method

2

GB/T 5009.124-2003

Determination of amino acids in food

3

GB/T 8314-2013

Determination of total free amino acids in tea

4

4NY 1429-2010

Amino acid containing water-soluble fertilizer

5

5GB/T 18246-2000

Determination of amino acids in feed

6

6NY/T 56-1987

Pre processing method for amino acid determination in grain

7

7QB/T 2409-1998

Determination of amino acid content in cosmetics

8

8QB/T 4356-2012

Determination of free amino acids in yellow wine

9

9SN/T 0930-2000

Determination of Total Amino Acids in Pollen for Import and Export

10

10GB/T 14924.10-2008

Determination of Amino Acids in Formula Feed for Experimental Animals

11

11GB/T 15399-1994

Method for the determination of sulfur-containing amino acids in feed - Ion exchange chromatography

12

12GB/T 15400-1994

Method for the determination of tryptophan in feed - spectrophotometric method

Due to limited space on the website, only a portion of the detection scope and items were listed. For more information, please call the toll free hotline at 400-101-7153 or click on online customer service, and an engineer will answer you one-on-one. The testing laboratory of Baijian Research Institute will provide you with high-quality testing services

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BAIJIAN testing process:

1. Telephone communication and confirmation of requirements

2. Recommend solutions and confirm quotations

3. Mail samples and arrange testing

4. Progress tracking and result feedback

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6. If urgent or priority processing is required

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