101 Dairy Product Testing

This standard specifies the determination method for aflatoxin B, aflatoxin B, aflatoxin G, and aflatoxin G (hereinafter referred to as AFT B, AFT B, AFT G, and AFT G) in food. The first method of this standard is isotope dilution liquid chromatography tandem mass spectrometry, which is applicable to the determination of AFT B, AFT B. AFT G, and AFT G in grains and their products, legumes and their products, nuts and seeds, oils and their crystallization, seasonings, infant formula foods, and infant auxiliary foods. The second method of this standard is the high-performance liquid chromatography column front street method, which is applicable to the determination of AFT B, AFT B, AFT G, and AFT G in grains and their crystals, beans and their products, nuts and seeds, oils and their products, seasoning crystals, infant formula foods, and infant auxiliary foods. The third method of this standard is the post column extraction method for high-performance liquid chromatography, which is applicable to AFT B. AFT B. AFT G and AFT G in grains and their products, legumes and their products, nuts and seeds, oils and their crystallization, seasoning crystals, infant formula food crystals, and infant auxiliary foods; Determination of. The fourth method of this standard is the enzyme-linked immunosorbent assay (ELISA) screening method, which is applicable to the determination of AFTB in grains and their products, beans and their products, nuts and seeds, oils and their preparations, seasoning crystals, infant formula food crystals, and infant auxiliary food crystals. The fifth method of this standard is thin layer chromatography, which is applicable to the determination of AFT B in grains and their crystallization, beans and their crystallization, nuts and seeds, oils and their products, and flavoring crystals. The first method is isotope dilution liquid chromatography-tandem mass spectrometry. The aflatoxin B, aflatoxin Bh, aflatoxin Gn, and aflatoxin Gn in the sample are extracted with acetonitrile aqueous solution or methanol aqueous solution. The extraction solution is diluted with a phosphate buffer solution containing 1% Triton X-100 (or Tween-20) (initially purified by a aflatoxin solid-phase purification column if necessary), purified and enriched by an immunoaffinity column, and the purification solution is concentrated After constant volume and filtration, it was separated by liquid chromatography, detected by Shenlian mass spectrometry, and quantified by isotope internal standard method. Unless otherwise specified, all reagents and materials used in this method are analytical pure, and the water is Grade 1 water as specified in GB/T 6682. 3.1 Reagent 3.1.1 Acetonitrile (CH, CN): Chromatically pure. 3.1.2 Methanol (CH, OH): Chromatically pure. 3.1.3 Ammonium acetate (CH, COONH,): Chromatically pure. 3.1.4 Sodium oxide (NaCI)

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