Yeast Extract Detection Inspection Certification Testing

The methods of yeast extract detection inspection and certification testing mainly include nutrient analysis, microbiological testing, heavy metal testing and impurity analysis. The following is a detailed description of these testing methods:

I. Nutrient Composition Analysis
Yeast extracts as a nutritional supplement, the content of its nutrients is critical. Commonly used methods for nutrient analysis include:

High performance liquid chromatography (HPLC): suitable for analyzing amino acids, vitamins and other compounds in yeast extracts.
Gas Chromatography-Mass Spectrometry (GC-MS): can be used to analyze volatile components in yeast extracts.
Spectrophotometry: can be used to determine the content of some specific nutrients, such as vitamins.
Second, microbial detection
Microbial contamination is an important factor affecting the hygiene and safety of yeast extracts. Commonly used microbial detection methods include:

Medium culture method: by cultivating the microorganisms in yeast extracts and observing their growth to determine the degree of contamination.
ATP bioluminescence detection method: utilizing the principle that ATP in microorganisms reacts with luciferin-luciferase to produce light to rapidly detect the number of microorganisms.
PCR technology: rapid and accurate detection of the presence of microorganisms by amplifying specific gene fragments of microorganisms.
III. Heavy metal detection
Heavy metal contamination is another safety issue to focus on in yeast extracts. Commonly used heavy metal detection methods include:

Atomic Absorption Spectrometry (AAS): determines the content of heavy metals by measuring the degree of absorption of specific light by atoms.
Inductively Coupled Plasma Mass Spectrometry (ICP-MS): It can simultaneously detect a variety of heavy metal elements with high sensitivity and high resolution.
IV. Impurity Analysis
Other chemical impurities that may exist in the yeast extract also need to be detected. Commonly used impurity analysis methods include:

Liquid chromatography-mass spectrometry (LC-MS): suitable for analyzing complex mixtures in yeast extracts, including unknown impurities.
Nuclear magnetic resonance spectroscopy (NMR): can be used to determine the structure and purity of compounds in yeast extracts.
V. Testing standards and requirements
The detection of yeast extracts needs to follow certain standards and requirements to ensure the accuracy and reliability of the test results. Common testing standards include:

GB/T 20886.2-2021 “Yeast Product Quality Requirements Part 2: Yeast Processed Products”: the definition of yeast extract, sensory requirements, physical and chemical indexes, hygiene indexes and other detailed provisions.
GB/T 23530-2009 Yeast Extracts: technical requirements, test methods, test rules, etc. for yeast extracts.
In addition, other relevant testing standards and methods can be selected according to specific testing needs and customer requirements.

VI. Testing Procedure
The testing procedure of yeast extract usually includes the following steps:

Sample preparation: take an appropriate amount of yeast extract sample to ensure its representativeness.
Pre-treatment: According to the specific test items and methods, the necessary pretreatment of the sample, such as dissolution, dilution and so on.
Detection and analysis: The samples are analyzed using appropriate detection methods to determine the content of each index.
Data Processing: Data processing and analysis of the test results to derive the specific values of the indicators.
Report writing: according to the test results and data processing results, write a test report, give the test conclusions and recommendations.
In summary, the testing and inspection of yeast extracts certification test method involves a number of aspects, including nutrient analysis, microbiological detection, heavy metal detection and impurity analysis. In the testing process, certain standards and requirements need to be followed to ensure the accuracy and reliability of the test results.