Drinking natural mineral water (microorganism) testing

The detection methods of auxiliary blood sugar-lowering health products mainly include animal experiments and human food trials.

1. Animal experiments
The main purpose of animal experiments is to observe the effects of test samples on animal-related indicators by giving them to normal animals and high blood sugar animal models caused by experimental methods, so as to evaluate their blood sugar-lowering effects.

Selection of experimental animals:
Usually, adult animals are selected, such as mice (weight about 26±2g) or rats (weight about 180±20g), and they should be of a single-sex.
There are also certain requirements for the number of animals in each group, such as 812 rats per group and 1015 mice per group.
Experimental design:
The experiment has three dose groups and one model control group, and a normal animal group that is given a high dose of the test sample is also set.
The test sample is generally given for 30 days, which can be extended to 45 days if necessary.
Experimental steps:
Normal animal experiment: Select healthy adult animals and group them according to their blood sugar level after fasting for 3~5 hours, and randomly select 1 control group and 1 dose group. The control group was given solvent, and the dose group was given high-dose concentration test samples for 30 consecutive days. Fasting blood glucose values ​​were measured (fasting was the same as before the experiment), and the blood glucose values ​​of the two groups of animals were compared.
Animal experiment on hyperglycemia model: Common hyperglycemia models include the islet injury hyperglycemia model and insulin resistance and lipid metabolism disorder model. Taking the islet injury hyperglycemia model as an example, the model was established by injecting alloxan (or streptozotocin), and the blood glucose value of 1025mmol/L was a successful hyperglycemia model animal. The hyperglycemia model animals were selected and grouped according to the blood glucose level after fasting for 35 hours, and 1 model control group and 3 dose groups were randomly selected (the difference between the groups was not more than 1.1mmol/L). The dose group was given different concentrations of test samples, and the model control group was given solvent for 30 consecutive days. Fasting blood glucose values ​​were measured (fasting was the same as before the experiment), and the blood glucose values ​​and blood glucose reduction percentages of animals in each group were compared.
Glucose tolerance test: animals in each group fasted for 35 hours, and the blood glucose level before glucose or medical starch (i.e., 0 hours) was measured. The dose group was given different concentrations of the test sample, and the model control group was given the same volume of solvent. After 15-20 minutes, each group was orally given glucose or medical starch, and the blood glucose level of each group was measured at 0.5 and 2 hours after glucose administration or 1 and 2 hours after medical starch administration. The changes in blood glucose level and the area under the blood glucose curve at each time point (0, 0.5, and 2 hours) after glucose or medical starch administration were observed in the model control group and the test sample group.
Experimental result judgment:
Scheme 1 (islet injury hyperglycemia model): If one of the two indicators of fasting blood glucose and glucose tolerance is positive, and has no effect on the fasting blood glucose of normal animals, it can be determined that the animal test results of the test sample's auxiliary hypoglycemic function are positive.
Scheme 2 (insulin resistance and lipid metabolism disorder model): If one of the two indicators of fasting blood sugar and glucose tolerance is positive, blood lipids (total cholesterol, triglycerides) are not significantly increased, and there is no effect on the fasting blood sugar of normal animals, it can be determined that the animal experiment results of the test sample's auxiliary hypoglycemic function are positive.
2. Human food trial
The purpose of the human food trial is to further observe the hypoglycemic effect and edible safety of the test sample in the human body on the basis of clinical treatment.

Subject selection:
The subjects are grouped according to the requirements of the random blind method and divided into a test group and a control group.
The subjects should have specific inclusion and exclusion criteria, such as age, gender, health status, blood sugar level, etc.
Experimental design:
The test group takes the test sample according to the recommended method of taking the medicine.
The control group can take a placebo or other methods on the basis of taking the medicine.
The test cycle is usually a period of time (such as 60 days), during which the clinical symptoms, signs, and various biochemical indicators of the subjects are observed and tested.
Test indicators:
Safety indicators: including general physical signs (such as spirit, sleep, diet, etc.), routine blood and urine tests, liver and kidney function tests, etc.
Efficacy indicators: including fasting blood sugar, 2-hour postprandial blood sugar, glycosylated hemoglobin (or glycosylated serum protein), blood lipid levels, etc.
Experimental results judgment:
There is no significant increase in the four indicators of fasting blood sugar, 2-hour postprandial blood sugar, glycosylated hemoglobin (or glycosylated serum protein), and blood lipids.
One of the two indicators of fasting blood sugar and 2-hour postprandial blood sugar is positive.
There is no effect on the health of the body, and it can be determined that the test sample has the function of assisting in lowering blood sugar.
It should be noted that the above test methods should be carried out under the guidance of professional institutions and professionals to ensure the accuracy and reliability of the test results. At the same time, for the detection of auxiliary blood sugar lowering health products, relevant laws, regulations and standards should also be followed to ensure their safety and effectiveness.