Detection of residual parameters of agricultural and veterinary drugs

This standard specifies the sample preparation method, high-performance liquid chromatography detection method, and liquid chromatography-mass spectrometry/mass spectrometry confirmation method for the detection of tetracycline residues in animal derived foods. This standard is applicable to the determination of residues of seven tetracycline veterinary drugs, including dimethylamine tetracycline, oxytetracycline, tetracycline, demethyl aureomycin, aureomycin, methyloxytetracycline, and doxycycline, in animal derived foods such as muscle, visceral tissue, aquatic products, and milk, as well as the determination of residues of dimethylamine tetracycline, epitetracycline, oxytetracycline, epitetracycline, tetracycline, demethyl aureomycin, epitetracycline, aureomycin, and methyloxytetracycline Liquid chromatography-mass spectrometry/mass spectrometry determination of 10 tetracycline residues in doxycycline. The clauses in the following documents are referred to as provisions of this standard through normative references. For dated reference documents, all subsequent amendments (excluding corrected content) or revisions are not applicable to this standard. However, parties to agreements based on this standard are encouraged to study whether the latest versions of these documents can be used. For undated references, the latest version applies to this standard. GB/T 6682 Specification and Test Methods of Water for Analytical Laboratory (GB/T 6682-1992, neq ISO 36961987) 3 Tetracycline antibiotics residues in principle samples are extracted with 0.1 mol/L Na, EDTA Mclvaine buffer solution (pH-4.0 ± 0.05), filtered and centrifuged, the supernatant is purified with HLB solid phase extraction column, determined by HPLC or liquid chromatography electrospray mass spectrometry, and quantified by external standard peak area method

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